Isolation and Identification of proteus spp from hatcheries and table eggs in Kerbala City

Abstract

This study set out to measure the frequency of Proteus spp. in hatcher settings gathered in Karbala City, as well as to identify and isolate these species. Bacteria belonging to the Magnoliaceae family, which are peritrichously flagellated, are distinguishable from other members of the order Enterobacterales by the multicellular differentiation phenomenon, which causes them to proliferate in intense and spectacular swarms on solid substrates. Three hundred samples were collected from different hatcheries in the city of Karbala, and culture tests such as (blood agar, MacConkey agar and XLD) and chemical tests such as (G stain, Catalase Oxidase test, Indole test, Cimone Citate test, Triple sugar ion (TSI) agar test and Urase production) were performed. Isolation was confirmed using the VITEK apparatus. The culture and chemical results showed the presence of bacteria Proteus spp .Out of the 300 isolates that were examined, the following categories of samples tested positive (14% of the total): newly 36.6% of hatched chicks, 13.3% of workers, 6.6% of doors, 0% of trays, 0% of incubators, 40% of chicken droppings, 0% of cardboard envelopes, 33.3% of the floor, 26.6% of fans, 20% of ventilation openings, and eggs and other developmental stages (0%) in conclusion P. mirabilis exhibits versatility bacterium recognized as a significant opportunistic uropathogen, particularly known for causing complicated in chicken hatcheries.