الملخص

The articular cartilage is a connective tissue with significant mechanical durability and elasticity function. Cartilage has limited self-repair capabilities due to its intrinsic low vascularity, which leads to a diminished replicative capability of chondrocytes. Cartilage defect is a debilitating joint disorder that affects the dog. In this study, we investigated the utilization of decellularized cartilage and Adipose tissue mesenchymal stem cells  associated with medical ozone for treating full-thickness cartilage defect in dogs. Methods: Twenty-five dogs were used. A full-thickness 8 mm diameter by (4) mm deep cartilage defect was performed in the left stifle joint of all dogs. They were divided into five equal groups. The induced defects of the first group were left without treatment. The defects of second group were filled with decellularized cartilage (DC). The defects of the third group were filled with (DC) then treated with Medical Ozone. The defects of the fourth group were filled with (DC) + (AT-MSCs). While the defects of the fifth group were filled with (DC) and (AT-MSCs) then treated with medical ozone. The dogs were euthanized at 56-day post operation. Results: The cartilage healing process of fifth group showed a new cartilage tissue formation in the peripheral and extended to the centre of the cartilage defect. The middle and superficial areas of the cartilage defect tend to be compact. Conclusion: Decellularized cartilage with both (AT-MSCs) and medical ozone which used in this present study as a treatment can effectively accelerate and promote the healing of cartilage defect of stifle joint in dogs