الملخص

Bone homeostasis and skeletal growth are mostly influenced by estrogen. Osteoclastic bone resorption in women is often accelerated by estrogen insufficiency following menopause. Examining the relationship between estrogen and the RANK/RANKL/OPG pathway as well as other biomarkers and their correlation with bone loss was the goal of the current investigation. Twenty female rabbits with ovariectomies were split into two groups at random: the ovariectomized group (OVX group) and the control group. Six weeks following ovariectomy, blood samples were obtained for biochemical analysis in order to determine the blood's calcium percentage and to evaluate the serum hormones estrogen, progesterone, LH, and FSH (OPG, RANKL, and RANk). Additionally, femur samples were obtained for histological sectioning analysis. According to the data, rabbits that had ovariectomy had significantly higher levels of RANk and RANkL, however OPG analysis showed that these rabbits' levels were significantly lower than those of the control group. According to the findings, rabbits with osteoporosis had lower serum levels of progesterone and estrogen than the control group. In contrast, the osteoporosis-affected rabbits' serum levels of FSH and LH were significantly higher than those of the control group.However, the calcium levels were higher than in the control group, according to the data. The rabbits' femur underwent histological examination, which showed osteoporosis, a high concentration of osteoclasts, and bone tissue reabsorption with thinner bone laminae. The results of the light microscope analysis of the bones showed that there were variations between the control and ovariectomized groups.