الملخص

The purpose of this study was to assess the prevalence and regional variation of Aflatoxin M1 (AFM1) levels in milk samples taken from five districts. Fifty milk samples were randomly collected from Al-Hamza, AL-Diwaniyha, Al-Sunniyah, Al-Shamiya and Al-Daghara regions from farms and markets of Al-Diwaniyah Governorate for the period between October 2025 and January 2026, in all (n = 10 each district) were examined. Every sample (100%) had a positive AFM1 test. Al-Hamza had mean quantities of 0.411 ± 0.041 ppb, while Al-Diwaniyah had mean values of 0.489 ± 0.048 ppb. Districts differed significantly, according to statistical analysis using one-way analysis of variance (ANOVA) (F(4,45) = 6.87, P = 0.0004). Al-Diwaniyah and Al-Sunniyah had considerably greater levels than Al-Hamza (P < 0.05), according to a post-hoc comparison using Duncan's multiple range test, whereas Al-Shamiya and Al-Daghara showed intermediate concentrations. AFM1 contamination levels may be influenced by management factors, feed storage techniques, and environmental conditions, according to the reported geographical variance. In order to lower exposure hazards and safeguard public health, these findings emphasize the necessity of ongoing monitoring programs and enhanced control mechanisms.