الملخص

In the recent decades, antimicrobial resistance (AMR), has been emerged as public health challenges. Since miss uses of antibiotics particularly in the developing countries. The threat of drug-resistant bacteria requires great efforts to develop ‎highly effective and safe bactericide. This study aimed to create silver nano particles (AgNPs) by reduction of silver nitrate with sodium citrate salt. The project included preparation of silver nano particles and characterization of AgNPs; UV–vis, XRD and AFM.  Besides, Fifty cloacal swabs were collected from broiler then, bacterial isolation and agar diffusion gel were done.  The result of AgNPs characterization were; UV-vis peak at 350 nm; X ray diffraction amorphous crystalline at size 7-10 nm; and AFM grain nanoparticles at size 27nm. The four bacterial spp. gained (50 isolates) were examined to the antibacterial profile of gram-positive bacterial isolates showed sensitivity(mm) to: Ciprofloxacin (21.52± 0.2), Gentamicin (20.54 ± 0.2), Levoxacine (20.57 ± 0.15), Norfloxacin (22.59 ± 0.2), Oxacillin (16.1± 0.2) and resistance to Tetracycline (0.00). Whereas, the antibacterial profile of gram-negative bacterial isolates displayed sensitivity (mm) to: Ciprofloxacin (20.2±0.2), Gentamicin (18.11± 0.1), Levofloxacin (18.22± 0.12), Norfloxacin (20.2±0.2), but resistance to Oxacillin and Tetracycline (00.0). The sensitivity profile of AgNP showed; (20.54 ± 0.2), (16.11± 0.1) against gram positive and gram-negative bacterial isolates respectively. Accordingly, AgNP shown sensitivity to gram positive and gram-negative bacteria included the resistance isolates. The AgNPs as antimicrobial agents holds promise for the use to sanitation process in poultry industry.